Figure 7. Salubrinal alleviated HIRI through phosphorylation of EIF2α. Old mice with Salubrinal pretreatment (O+SAL) or without (O) were treated as indicated. (A) The phosphorylated EIF2α, cleaved PARP (CL PARP), Parkin and TOMM20 protein levels were determined by western blot analysis from indicated mice. (B) Representative histology of liver by H&E staining (top panel) and TUNEL staining (bottom panel) from indicated groups. (C) Representative reactive oxygen species generation by MitoSOX Red staining and quantification of MitoSOX red fluorescence intensity. (D) Measurements of serum AST and ALT from indicated groups. The data are expressed as mean ± SD. Statistical comparisons were performed with t-test. *P < 0.05 vs. the indicated group. Scale bar: 50μm.