Figure 1. Functional Sirt6 is expressed in mouse cornea. (A) Protein was extracted from the corneas of WT and Sirt6-/- mice and Sirt6 protein expression was assessed by Western blot analysis. α-Tubulin served as loading control. (B, C) Immunostaining of Sirt6 (green), H3K9 and H3K56 (red) in corneal cryosections from 2 month-old WT and Sirt6 KO mice. Blue: DAPI staining for nuclei. Scale bar: 50μm. (D) Sirt6 protein expression in young (2 months old) and old (12 months old) mice cornea. Two corneas served as one specimen. *p<0.05, n=4 mice.