Figure 3. Pathological changes of the cornea of Sirt6 KO mice after injury. 2.0 mm central corneal epithelial debridement wounds were induced in 8-week-old WT and Sirt6 KO mice and eyes were collected 3 weeks after injury. (A) Histological appearance of corneas with H&E staining at 200X magnification (upper panel) and 400X magnification (lower panel). (B) Immunostaining of corneal epithelium marker keratin 12, and the keratinization-related protein loricrin (red) in corneal cryosections from WT and Sirt6 KO mice. Blue: DAPI staining for nuclei. Scale bar: 50 μm.