Figure 5. Sirt6 knockdown induces inflammation in vitro. Human primary corneal epithelial cells (HCEs) were transiently transfected with control siRNA (con) or Sirt6 siRNA. 24 hours after transfection, HCEs were treated with different concentrations of IL-1β (0.3 and 1 ng/ml), TNF-α (0.1 and 1 ng/ml) or vehicle (Vec) for 24 hours. (A) qPCR analysis of inflammatory gene expression. *P<0.05 compared to relevant control siRNA; #p<0.05 compared with vehicle, n=3. (B) Knockdown of Sirt6 by siRNA in HCEs was confirmed by qPCR (upper panel) and Western blot analysis (lower panel). *p<0.05 compared with control siRNA, n=3 times of independent experiment.