Figure 7. Sirt6-/- mice develop corneal plaques with infiltration of inflammatory cells and neovascularization as they age. Eyes were collected from 7-month-old Sirt6 KO mice and their littermate WT mice. (A) Images of the corneas and H&E staining of cross-sections of eyes from WT and Sirt6 KO mice (n=6 mice). Scale bar: 50 μm. (B) Immunostaining of keratin 12 (red) and loricin (green). Scale bar: 100 μm. (C) Immunostaining for hematopoietic cell markers such as CD45 for lymphoid cells (monocytes) (green), Iba1 for activated macrophages, MPO for neutrophils, CD3 for T cells, CD19 for B cells and CD31 for neovascularization in 7 month-old WT and Sirt6 KO corneas. Nuclei are counterstained with DAPI (blue). Scale bar: 100 μm.