Figure 1. Age-related changes in the activity of the Nrf2/HO-1 pathway. (A) Immunohistochemistry of Nrf2 in the ovaries of hens aged 90, 150, 280 and 580 days, scale bar: 10 μm, black arrowheads: Nrf2 located in the nucleus. (B) Age-related changes in relative expression levels of Nrf2, pNrf2, Keap1, HO-1 and NQO1. (C) Age-related changes in transcription levels of Nrf2/HO-1 downstream genes: Gclc, Gclm, Glrx, Gpx1, Txn, Txnrd. Values are expressed as the means±s.e.. The relative abundance of each transcript was normalized to a β-actin and expressed as fold change over D90 ovaries. Different lowercase letters indicate significant differences (P < 0.05).