Research Paper Volume 10, Issue 8 pp 2037—2050

The long non-coding RNA FLJ46906 binds to the transcription factors NF-κB and AP-1 and regulates expression of aging-associated genes

Figure 1. Expression of the lncRNA FLJ46906 increases with aging in fibroblasts. (A) The expression of FLJ46906 in fibroblasts from older donors (63, 68, and 70 years old, aged in-vivo), as measured by qPCR, is 2.8 fold higher than in fibroblasts from young donors (18, 23, and 27 years old; n = 3 (triplicate samples from each donor), mean ± SD, *p < 0.05). (B) Neonatal fibroblasts aged in-vitro by longterm culture show increasing expression of FLJ46906 with increasing population doublings (PD), as measured by qPCR (n = 3 (cells from triplicate tissue culture dishes), mean ± SD, *p < 0.05, **p < 0.01).