Research Paper Volume 10, Issue 8 pp 2062—2078

LncRNA BANCR promotes tumorigenesis and enhances adriamycin resistance in colorectal cancer

Figure 5A-F. BANCR acted as a molecular sponge of miR-203 to sequester miR-203 away from CSE1L in CRC cells. (A) Predicted binding sites between BANCR and miR-203, and mutant sites in Mu-BANCR reporter. (B and C) The effects of miR-203 overexpression on luciferase activity of WT-BANCR and Mu-BANCR reporters were detected in LoVo and HCT116 cells. (D) Putative binding sequences between miR-203 and CSE1L 3’UTR, and mutant sites in CSE1L 3’UTR-Mu reporter. (E and F) The effects of miR-203 overexpression on luciferase activity of CSE1L 3’UTR-WT and CSE1L 3’UTR-Mu reporters were determination in LoVo and HCT116 cells.