Research Paper Volume 10, Issue 8 pp 2113—2121

MiR-146b inhibits autophagy in prostate cancer by targeting the PTEN/Akt/mTOR signaling pathway

Figure 3. MiR-146b directly targeted the 3’-UTR of PTEN mRNA. (A) Schematic representation of the mature miR-146b sequence, putative miR-146b target site in the 3’-UTR of PTEN mRNA. (B) Overexpression of miR-146b markedly decreased the relative luciferase activity in the WT 3’-UTR of PTEN mRNA, while the mutated 3’-UTR of PTEN was insensitive to miR-146b overexpression. (C-D) MiR-146b mimic transfection decreased the expression of PTEN significantly. (**P < 0.01).