Figure 6. Oltipraz ectopically activates Nrf2 signaling and increases lifespan of CS exposed flies. CS exposure impacts the expression of GFP in Nrf2 reporter lines (A-D). 3rd instar ARE-GFP larvae were left untreated (A) or exposed to CS (B). GstD2-GFP animals left untreated (C) or CS exposed (D). Impact of oltipraz on the activation of Nrf2 signaling in 3rd instar larvae (E-H). 3rd instar ARE-GFP larvae were left untreated (E) or exposed to oltipraz (F). GstD2-GFP animals left untreated (G) or treated with oltipraz (H). Effect of oltipraz on the transcript levels of canonical Nrf2 target genes (J, N=3, means ±S.D., n = 5, p > 0.01 for gstd5). Lifespan analysis of CS exposed animals (daily doses) that were left otherwise untreated (red symbols) or that were chronically confronted with oltipraz (blue symbols) (p<0.001, n = 5).