Research Paper Volume 10, Issue 8 pp 2136—2147

p62 /SQSTM1 coding plasmid prevents age related macular degeneration in a rat model

Figure 5. p62DNA abolished migration of Iba1+ and Cd68+ positive cells (microglia and macrophages) to the outer nuclear layer in 13.5-month-old OXYS rats. (A) Representative images of Iba1 (green) and Cd68 (red) immunostaining in OXYS rats treated with PBS or p62DNA at 3 and 13.5 months of age. The distribution of Iba1+ (B) and Cd68+ cells (C) in various layers of the retina: ganglion and inner plexiform layers (GCL+IPL, black), inner nuclear and outer plexiform layers (INL+OPL, red) and outer nuclear layer (ONL, blue). Scale bar: 50μm. ONL: outer nuclear layer; INL: inner nuclear layer; GCL: ganglion cells layer. *p < 0.05, statistically significant effect of p62DNA. Data are presented as mean ± SEM.