Figure 2. Signaling downstream of estrogen in the mitochondria of the cerebral cortex across age and gender. Representative western blots for each protein of interest and a representative Ponceau stain as a load control (A). Graphical depiction of the fold change for p-cx43 comparing age (B), p-cx43 comparing gender (C), cx43 (D), estrogen receptor beta (E), PKC (F), ATP synthase (G). Error bars = SEM. 4W = 4 weeks of age; 3M = 3 months of age; 9M = 9 months of age; 12M = 12 months of age. Pink = female; blue = male. ANOVA with Tukey posthoc, * = P<0.05.