Figure 5. Signaling downstream of estrogen in the amygdala across age and gender. Representative western blots for each protein of interest and a representative Ponceau stain as a load control (A). Graphical depiction of the fold change for p-PKC (B), PKC (C), p-ERK (D), ERK (E), p-cx43 (F), cx43 (G), beta-actin (H). Error bars = SEM. 4W = 4 weeks of age; 3M = 3 months of age; 9M = 9 months of age; 12M = 12 months of age. Pink = female; blue = male. ANOVA with Tukey posthoc, * = P<0.05.