Research Paper Volume 10, Issue 8 pp 2170—2189

Exposure to Zinc oxide nanoparticles during pregnancy induces oocyte DNA damage and affects ovarian reserve of mouse offspring

Figure 2. Apoptotic and DNA damage markers in nZnO treated fetal oocytes in vitro. (A-D) Representative q-RT-PCR and WB analyses of Bax, Bcl-2 and Mvh of ovarian tissues cultured for 6 days; Actin or Mvh was used as housekeeping gene and loading control, respectively. (E) TUNEL-staining of the ovarian tissues after 6 days of culture with nZnO. (F) Number of TUNEL-positive total cells, TUNEL-positive oocytes and TUNEL-positive granulosa cells per section. (G) Co-immunostaining of DNA damage marker γH2AX (green) and germ cell marker MVH (red) in ovary sections after 6 day nZnO exposure, Hoechst 33342 (blue) was used for nuclei staining. (H) Percentage of MVH positive oocytes also stained for γH2AX. (I) Number of MVH positive oocytes/ovary.