Figure 5. nZnO expose decrease the oocyte number and affect oocyte specific gene expressionin 3 dpp ovaries. (A) Representative immunofluorescence pictures of MVH stained oocytes in sections of 3 dpp ovaries; Hoechst 33342 was used for nuclei staining; note oocytes within PF or in Cyst. (B) Number of oocytes per ovary. (C) Percentage of oocytes in PF or Cyst. (D) Representative WB of the indicated proteins in 3 dpp ovaries; (E) Quantitative RT‐PCR for Mvh and Lhx8 mRNA levels in 3 dpp ovaries, Actin or Mvh was used as housekeeping gene and loading control. (F) IF for NOBOX (red) and fluorescence intensity of NOBOX in oocytes of control and nZnO exposed ovaries in 3 dpp ovaries; Hoechst 3342 (blue) was used for nuclei staining.