Figure 4. Down-regulation of CD34 impairs endothelium function in vitro. (A) CD34 protein levels in treated HUVEC cells detected by Western blotting. (B) Migration evaluated by transwell experiment in HUVEC cells. (C) Tube formation determined on Matrigel in HUVEC cells. (D) Apoptosis measured by Annexin V/PI flow cytometric analysis in HUVEC cells. (E) Proliferation detected by EdU incorporation assays in HUVEC cells. Data are representative of four experiments, n=4. Data are expressed as mean ± SEM.