Research Paper Volume 10, Issue 9 pp 2498—2510

Sirt1 enhances tau exon 10 inclusion and improves spatial memory of Htau mice

Figure 1. Resveratrol enhances Sirt1 expression and suppresses 4R-tau expression. (A, B) 5-month old Htau mice were treated with resveratrol for 7 months. The levels of 3R-tau, 4R-tau and total tau in the brains were analyzed by western blot developed with anti-Sirt1 (A) and anti-3R-tau and anti-4R-tau (B). The data are presented as mean ± S.D. (n=3-5) and analyzed by student t-test. **, p<0.01. (C) pCI/SI9-SI10 was transfected into HEK-293T cells and then the cells were treated with 0, 25, 50 and 100 μM of resveratrol for 48 h. Total RNA was extracted and alternative splicing of tau exon 10 was analyzed by RT-PCR. Ratio of exon 10 inclusion/exon 10 exclusion was plotted against the resveratrol concentration.