Research Paper Volume 10, Issue 10 pp 2585—2605

H3K9me3 and H4K20me3 represent the epigenetic landscape for 53BP1 binding to DNA lesions

Figure 2. Quantification of western blot data on selected histone markers in non-irradiated and γ-irradiated cells. Using ImageJ and ImageQuant TL software, the levels of the following proteins (originated from Fig. 1A, B) were quantified: H3K9me1, H3K9me2, H3K9me3, H3K9ac, H4K20me2, H4K20me3, H4K20ac, γH2AX, HP1β, 53BP1, and MDC1. The levels of modified histones were normalized to those of total H3 histones, and those of DDR-related proteins were normalized to those of α-tubulin. Quantification was performed in the following samples: (A) HAP1 wt and HAP1 mutant cells and (B) wt MEFs and Suv39h1/h2-deficient fibroblasts (MEFs).