Research Paper Volume 10, Issue 10 pp 2606—2623

Circular RNA circUBXN7 represses cell growth and invasion by sponging miR-1247-3p to enhance B4GALT3 expression in bladder cancer

Figure 3. CircUBXN7 exerted a tumor suppressive role in BC cells. (A) Schematic of the silencing sites of circUBXN7 and linear UBXN7. (B) Relative expression of circUBXN7 and UBXN7 mRNA after knockdown of circUBXN7 or UBXN7 examined by qRT-PCR. (C) Relative expression of circUBXN7 and UBXN7 mRNA after overexpression of circUBXN7. (D) MTS cell viability assay of T24 cells treated with si-circUBXN7. (E) Cell clone numbers were counted when circUBXN7 was silenced in T24. (F) Overexpression of circUBXN7 inhibited cell growth in UM-UC-3 cells detected by MTS assay. (G) CircUBXN7 overexpression reduced clone numbers of UM-UC-3 cells tested by colony formation assay. (H) Silencing of circUBXN7 enhanced cell migration of T24 cells performed by wound healing assay. Scale bar, 500 μm. (I) Knockdown of circUBXN7 increased the invasive ability of T24 cells as detected by transwell Matrigel invasion assay. Scale bar, 200 μm. (J) CircUBXN7 overexpression impaired migratory capacity of UM-UC-3 cells. (K) CircUBXN7 overexpression repressed invasive ability in UM-UC-3 cells. *P<0.05, **P<0.01.