Research Paper Volume 10, Issue 10 pp 2606—2623

Circular RNA circUBXN7 represses cell growth and invasion by sponging miR-1247-3p to enhance B4GALT3 expression in bladder cancer

Figure 5. CircUBXN7 reversed the miR-1247-3p-induced increase in proliferation, migration and invasion in BC cells. (A) Relative expression of miR-1247-3p in BC cell lines was examined by qRT-PCR. (B) qRT-PCR analysis of miR-1247-3p expression in BC and normal tissues. (C and D) MTS assay indicated that miR-1247-3p elevated cell viability of BC cells and the effect was abolished by circUBXN7. (E and F) Colony formation assay showed that miR-1247-3p increased clone numbers of BC cells and the effect was relieved by circUBXN7. (G-I) Wound healing assay revealed that miR-1247-3p enhanced the migratory ability of BC cells and this effect was abrogated by circUBXN7. Scale bar, 500 μm. (J) Transwell Matrigel invasion assay demonstrated that miR-1247-3p augmented the invasive capacity of BC cells and this effect was eliminated by circUBXN7. Scale bar, 200 μm. *P<0.05, **P<0.01, ***P<0.001.