Research Paper Volume 10, Issue 10 pp 2606—2623

Circular RNA circUBXN7 represses cell growth and invasion by sponging miR-1247-3p to enhance B4GALT3 expression in bladder cancer


Figure 6. CircUBXN7 antagonized miR-1247-3p-mediated repression of B4GALT3 expression. (A) The potential binding sites between miR-1247-3p and B4GALT3 was predicted by Targetscan. The red part indicates the mutated base. (B) Dual-luciferase reporter assay showed that B4GALT3 was directly targeted by miR-1247-3p. (C) The effect of si-circUBXN7 on the mRNA level of B4GALT3 was determined by qRT-PCR. (D and E) CircUBXN7 reversed the suppressive effect of miR-1247-3p on B4GALT3 expression at the mRNA level. (F and G) CircUBXN7 neutralized miR-1247-3p-induced inhibition on B4GALT3 expression at the protein level. (H and I) MTS assay showed that the proliferation-promoting effect of si-B4GALT3 was reversed by circUBXN7. (J and K) Transwell Matrigel invasion assay indicated that circUBXN7 counteracted the invasive ability of BC cells transfected with si-B4GALT3. *P<0.05, **P<0.01.