Figure 1. Autophagic flux and related genes increase with a high glucose diet. (A) Lifespan determined by Kaplan-Meier analysis of N2 wild type animals treated with a high glucose diet showed a decrease in lifespan compared to that of untreated animals. (B) Expression of selected autophagic and lysosomal genes measured by quantitative PCR (qPCR) that showed increased mRNA with high glucose. The relative expression of each gene was normalized to that of endogenous 18S rRNA. (C) Representative Western blot shows an increase in the band of mFP from the dimeric dFP-LGG-1 when worms were subjected to a high glucose diet. (D) The mFP/dFP-LGG-1 ratio indicates an increase in autophagic flux compared to that in normal conditions. *** p < 0.001; ** p < 0.01, Error bars represent ± SEM, t test with Bonferroni’s post hoc test using GraphPad Prism.