Figure 2. HLH-30 is activated by a high glucose diet. (A and B) Representative confocal images and quantitation, respectively, showing the nuclear localization of HLH-30 with the high glucose diet (green dots). Nuclei were visualized by DAPI staining (blue dots). (C) shows the expression level of hlh-30 mRNA that was increased in wild type nematodes treated with a high glucose diet. (D) mRNA expression of putative autophagy-related and lysosomal target genes in the N2 and hlh-30 (tm1978) mutant strains. Data represent the mean ± SEM of three independent experiments; *** p < 0.001, one-way ANOVA with Bonferroni’s post hoc test using GraphPad Prism.