Research Paper Volume 10, Issue 10 pp 2657—2667

A high glucose diet induces autophagy in a HLH-30/TFEB-dependent manner and impairs the normal lifespan of C. elegans

Figure 4. PPI and/or PP2A might regulate HLH-30 activation. (A) Confocal images of HLH-30::GFP worms treated with high glucose and TAX-6 interference by RNAi did not affect the nuclear localization of HLH-30, whereas pharmacologic addition of okadaic acid (120 nM) prevented it with a high glucose diet. Quantification is given in (B). Nuclei were labeled with DAPI (blue dots), (C) qRT-PCR analysis of tax-6 mRNA with or without high glucose after treatment with control (empty vector) or tax-6 RNAi. p-value (***p< 0.001, **p < 0.01). Error bars represent ± SEM, one-way ANOVA with Bonferroni’s post hoc test using GraphPad Prism.