Figure 4. STZ-induced DE is alleviated by RAP and exacerbated by CQ. Rapamycin (RAP) or Chloroquine (CQ) were administered to STZ-treated rats. (A) Fasting glycemia in rats. (B) Serum insulin content. (C) IPGTT 6 weeks after STZ. (D) Representative immunohistochemistry for insulin in the tail pancreas of rats 6 weeks after STZ. (E) Beta-cell mass 6 weeks after STZ. (F-H) The development of DE was assessed by an MWM assay. (F) The escape latency. (G) The percentage of time spent in the target quadrant. (H) The number of times that the rats crossed the platform. (I-L) Assessment of brain degradation markers. (I) Brain Malondialdehyde. (J) Catalase. (K) Superoxidase anion-positive cells. (L) Nitrites. *p<0.05. NS: non-significant. N=10. Scale bars are 100µm.