Research Paper Volume 10, Issue 10 pp 2783—2799

Ubiquitin-specific protease 4 promotes metastasis of hepatocellular carcinoma by increasing TGF-β signaling-induced epithelial-mesenchymal transition

Figure 4. USP4 knockdown blocked the metastasis of HCC cell in vivo. (A) Representative images of hepatic metastatic nodules originated from SK-Hep1-shRNA/USP4 cells and negative control cells, and the quantitative analysis of hepatic metastatic nodules from each group (* P < 0.05). (B) The hematoxylin and eosin (HE) staining evaluated the pathological character of hepatic metastatic nodules originated from SK-Hep1-shRNA/USP4 cells and negative control cells (magnification ×400). (C) Immunohistochemical staining (IHC) examined the expression of USP4 in hepatic metastatic nodules originated from SK-Hep1-shRNA/USP4 cells and negative control cells (magnification ×400). (D) Representative images of metastatic nodules in lung originated from negative control cells.