Figure 2. Autophagic vacuoles (AV)s and LC3 expression in yHUVECs and sHUVECs. (A) TEM images of AVs (arrowheads) in yHUVECs and sHUVECs (left); AVs were quantified per cell area (right), n = number of cells analyzed. Asterisk near a Weibel Palade body. Magnification, 19 K. (B) Western blot and densitometric analysis of LC3-II/LC3-I ratio fold change to P3 (yHUVECs) during replicative senescence. Data were normalized to β-actin. Data are mean ± SD of three independent experiments. * t-test p < 0.05; ** t-test p < 0.01.