Research Paper Volume 10, Issue 10 pp 2855—2873

The mitomiR/Bcl-2 axis affects mitochondrial function and autophagic vacuole formation in senescent endothelial cells

Figure 2. Autophagic vacuoles (AV)s and LC3 expression in yHUVECs and sHUVECs. (A) TEM images of AVs (arrowheads) in yHUVECs and sHUVECs (left); AVs were quantified per cell area (right), n = number of cells analyzed. Asterisk near a Weibel Palade body. Magnification, 19 K. (B) Western blot and densitometric analysis of LC3-II/LC3-I ratio fold change to P3 (yHUVECs) during replicative senescence. Data were normalized to β-actin. Data are mean ± SD of three independent experiments. * t-test p < 0.05; ** t-test p < 0.01.