Figure 6. Effects of transfection of the miRNA mimics 34a, 146a, and 181a on yHUVECs. yHUVECs were incubated for 24 h with the miRNA mimics (miR-34a, -146a, and -181a) or with a miRNA mimic negative control (CTR) before analysis. The percentage of transfected yHUVECs showing (A) mPTP opening, (B) annexin V positivity and active casp-3, and (E) casp-1 were evaluated by flow cytometry. (C) Western blot and densitometric analysis of Survivin expression in transfected yHUVECs. Survivin expression is reported as fold change in miRNA mimic-transfected cells vs CTR. (D) Bcl-xL mRNA relative expression in transfected yHUVECs. Data are mean ± SD of three independent experiments. * t-test p < 0.05, ** t-test p < 0.01, *** t-test p < 0.001.