Figure 4. Sirt3 rescues mitochondrial function impaired by Aβ-42. (A) HT22 cells were successfully transfected (green) with vector, Sirt3 cDNA or shRNA. The percentage of transfection was analyzed on Accuri C6 Flow Cytometer. (B) Before transfection, the percentage was 0 (negative control); (C) After transfection, there were 94.7% cells transfected. Sirt3 protein levels in transfected cells were further confirmed by using Western blot. (D) A representative Western blot of Sirt3. β-actin was used as an internal control. (E) In transfected cells, Sirt3 protein levels were measured with normalization with β-actin. (F) Sirt3 deacetylation activity (the ratio of fluorescent intensity to total protein) and (G) ATP levels were tested in isolated mitochondria. N= 6 per group, *p< 0.05, **p< 0.01 vs. vector; #p< 0.05, ##p< 0.01 vs. respective treated group.