Research Paper Volume 10, Issue 10 pp 2541—2543

Figure 1. Untouched CD8⁺ T cells from elderly mice show reduced thapsigargin (TG)-induced Ca²⁺ signals. (A) Fura2-AM based Ca²⁺ Imaging with 1 μM TG as stimulus applied in the presence of 0.5 mM [Ca²⁺]_ext of CD8⁺ T cells (combined Ca²⁺ protocol) from adult (black, n = 5) and elderly (red, n = 7) mice. The scatter dot plot in (B) displays the corresponding statistics of Ca²⁺ influx peak and Ca²⁺ plateau and in (C) the corresponding influx rates. (D) Ca²⁺ Imaging with 1 μM TG applied in the absence of [Ca²⁺]_ext before re-addition of 0.5 mM Ca²⁺ (re-addition protocol) of CD8⁺ T cells from adult (black, n = 5) and elderly (red, n = 7) mice. The scatter dot plot in (E) displays the corresponding statistics of Ca²⁺ influx peak and Ca²⁺ plateau and (F) the corresponding influx rates. Ca²⁺ signalling curves are presented as mean ± SEM. Scatter dot plots are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.