Figure 1. hAFSCs derived from amniotic fluid of physiological pregnancy: comparison of the proliferation capability and stemness profile. (A) The graph shows the cumulative population doubling (CPD) calculated up to the 7th passage in culture for all the samples. (B) Population doubling time (PDT) values of all the samples, evaluated at the third passage in culture. (C) Quantitative analysis by flow cytometry of four representative samples for the expression of the surface markers: CD44, CD73, CD29, CD105, CD90. (D) Graph showing the cytofluorimetric analysis of Nanog, Oct4, Sox2, SSEA4 expression among the three groups. ***P < 0.0001; **P < 0.01 = significantly different from group I, ###P < 0.0001; ##P < 0.01 = significantly different from group II.