Figure 1. Nrf2 decline in old mouse oocytes. Western blot analysis revealed a reduced Nrf2 expression in mouse oocytes from aged females compared with those from young controls. Actin served as a loading control throughout. Band intensity was calculated using ImageJ software, the ratio of Nrf2/Actin expression was normalized and values are indicated. Data are expressed as the mean ± SD, *P<0.05 vs. control.