Research Paper Volume 10, Issue 10 pp 2991—3004

Sirt1/Nrf2 pathway is involved in oocyte aging by regulating Cyclin B1

Figure 2. Cellular distribution of Nrf2 during oocyte meiosis. (A) Immunofluorescent staining was employed to show the subcellular localization of Nrf2. Green, Nrf2; Blue, chromatin. A total of 30 oocytes were examined for each group. Scale bar, 20 μm. (B) Immunofluorescent staining for co-localization of α-tubulin with Nrf2 in mouse oocytes. This shows the co-localization of Nrf2 with spindles in mouse oocyte. Red, Nrf2; green, tubulin; blue, chromatin. Scale bar, 20 μm.