Research Paper Volume 10, Issue 12 pp 3683—3701

A functional variant of SMAD4 enhances macrophage recruitment and inflammatory response via TGF-β signal activation in Thoracic aortic aneurysm and dissection

Figure 3. rs12455792 C>T variant promote the chemotaxis of MØ to HASMCs. (A) Representative fluorescence images of MØ migrating to HASMCs transfected with siRNA-NC or siRNA-SMAD4 in transwell assay. (B) The quantitative analysis of MØ cell numbers in different groups, n=5/group. (C) Representative fluorescence images of MØ migrating to HASMCs carrying rs12455792 CC, CT or TT genotypes in transwell assay. (D) The quantitative analysis of MØ cell numbers in different groups, n=5/group. Red: Dil-prestained MØ; Green: Dio-prestained HASMCs; Blue: DAPI. Data are means ± SD. **P<0.01, ns: not significant. Scale bar: 50μm.