Figure 2. BCAR4 increases androgen-independent PC cell growth and migration in vitro. (A) RT-qPCR for BCAR4 in 7 commonly used PC cell lines. Androgen receptor (AR)- expressing PC346 expressed the least level of BCAR4, while AR-null DU145 expressed the highest level of BCAR4. (B) We overexpressed BCAR4 in PC346 cells, and examined BCAR4 levels by RT-qPCR. (C-E) BCAR4-transfected cells and control scrambled-transfected cells were treated with null, or R1881, a recombinant androgen, with/without an androgen antagonist OH-flutamide (OHF). (C) Cell growth was examined in an CCK-8 assay. (D-E) Cell migration potential was assessed in a transwell assay, shown by quantification (D), and by representative images (E). *p<0.05. NS: non-significant. N=5. Scale bars were 20µm.