Figure 3. Loss of BCAR4 reduces basal PC cell growth and migration but regains androgen-sensitivity of PC cells in vitro. (A) We depleted BCAR4 in DU145 cells by shRNA and examined BCAR4 levels by RT-qPCR. (B-D) The shBCAR4-transfected cells and control scrambled-transfected cells were treated with null, or R1881, with/without OHF. (B) Cell growth was examined in an CCK-8 assay. (C-D) Cell migration potential was assessed in a transwell assay, shown by quantification (C), and by representative images (D). *p<0.05. NS: non-significant. N=5. Scale bars were 20µm.