Research Paper Volume 10, Issue 12 pp 3702—3712

BCAR4 activates GLI2 signaling in prostate cancer to contribute to castration resistance

Figure 4. BCAR4 activates GLI2-signaling pathways in PC cells. (A) GLI2 reporter luciferase assay on scrambled or BCAR4 transfected PC346 cells. (B) RT-qPCR on GLI2 downstream genes IL-6, RPS3, TGFβ1 and MUC5AC in scrambled or BCAR4 transfected PC346 cells. (C) GLI2 reporter luciferase assay on scrambled or shBCAR4 transfected DU145 cells. (D) RT-qPCR on GLI2 downstream genes IL-6, RPS3, TGFβ1 and MUC5AC in scrambled or shBCAR4 transfected DU145 cells. (E-H) Correlation between BCAR4 levels and IL-6 (E), RPS3 (F), TGFβ1 (G), and MUC5AC (H) in the 40 CRPC specimens. *p<0.05. N=5 for A-D and N=40 for E-H.