Research Paper Volume 10, Issue 12 pp 3702—3712

BCAR4 activates GLI2 signaling in prostate cancer to contribute to castration resistance

Figure 5. Depletion of GLI2 abolishes the effects of BCAR4 on growth and migration of PC346 cells. (A) We used shGLI2 to deplete GLI2 in BCAR4-transfected PC346 cells and did RT-qPCR for BCAR4 and GLI2. Transfection with shGLI2 significantly decreased GLI2 and GLI2 downstream genes IL-6, RPS3, TGFβ1 and MUC5AC, but did not alter BCAR4 levels. (B-C) These cells (shGLI2 and BCAR4-transfected cells, BCAR4-transfected cells and control scrambled-transfected cells) were treated with null, R1881, with/without OHF. (B) Cell growth was examined in an CCK-8 assay (C) Cell migration potential was assessed in a transwell assay. *p<0.05. NS: non-significant. N=5.