Figure 5. Depletion of GLI2 abolishes the effects of BCAR4 on growth and migration of PC346 cells. (A) We used shGLI2 to deplete GLI2 in BCAR4-transfected PC346 cells and did RT-qPCR for BCAR4 and GLI2. Transfection with shGLI2 significantly decreased GLI2 and GLI2 downstream genes IL-6, RPS3, TGFβ1 and MUC5AC, but did not alter BCAR4 levels. (B-C) These cells (shGLI2 and BCAR4-transfected cells, BCAR4-transfected cells and control scrambled-transfected cells) were treated with null, R1881, with/without OHF. (B) Cell growth was examined in an CCK-8 assay (C) Cell migration potential was assessed in a transwell assay. *p<0.05. NS: non-significant. N=5.