Research Paper Volume 10, Issue 12 pp 3702—3712

BCAR4 activates GLI2 signaling in prostate cancer to contribute to castration resistance

Figure 6. Overexpression of GLI2 abolishes the effects of shBCAR4 on growth and migration of DU145 cells. (A) We overexpressed GLI2 in shBCAR4-transfected DU145 cells and did RT-qPCR for BCAR4 and GLI2. Transfection with GLI2 significantly increased GLI2 but did not alter BCAR4 levels. (B-C) These cells (GLI2 and shBCAR4-transfected cells, shBCAR4-transfected cells and control scrambled-transfected cells) were treated with null, R1881, with/without OHF. (B) Cell growth was examined in an CCK-8 assay (C) Cell migration potential was assessed in a transwell assay. *p<0.05. NS: non-significant. N=5.