Figure 6. Overexpression of GLI2 abolishes the effects of shBCAR4 on growth and migration of DU145 cells. (A) We overexpressed GLI2 in shBCAR4-transfected DU145 cells and did RT-qPCR for BCAR4 and GLI2. Transfection with GLI2 significantly increased GLI2 but did not alter BCAR4 levels. (B-C) These cells (GLI2 and shBCAR4-transfected cells, shBCAR4-transfected cells and control scrambled-transfected cells) were treated with null, R1881, with/without OHF. (B) Cell growth was examined in an CCK-8 assay (C) Cell migration potential was assessed in a transwell assay. *p<0.05. NS: non-significant. N=5.