Research Paper Volume 10, Issue 12 pp 3713—3735

Reduced production of laminin by hepatic stellate cells contributes to impairment in oval cell response to liver injury in aged mice

Figure 6. The OC-supporting function of HSCs relies on DNA-PK pathway. (A) Quantitative Real-time PCR analysis of HR and NEHJ components in HSCs freshly isolated from young (2m) and aged (24m) mice with DDC diet (n=4, *** p < 0.001). (B) The expression of α-SMA (red) and laminin (green) in etoposide (0.1 μM) and DMNB treated JS1 cells was detected by confocal. MFI of laminin was quantified (n=5, ** p < 0.01). (C) Quantitative Real-time PCR showed that the expression of laminin isoforms in etoposide (0.1 μM) and DMNB treated JS1 cells (n=6, * p < 0.05, ** p <0.01). (D) Proliferation of OCs co-cultured with JS1 cells pretreated with etoposide, DMNB or laminin was compared by BrdU analysis. BrdU+ cell percentage was quantified (n=4, * p < 0.05). (E) Western blot analysis of indicated proteins in HSCs isolated from wild type (WT) and Ku80 knockout (KO) mice fed with DDC. (F) Immunofluorescence staining for EpCAM+ cells (green) in DDC-fed wild type (WT) versus Ku80 knockout mice (KO). Quantification of EpCAM+ cells was shown (n=5, ** p <0.01).