Research Paper Volume 10, Issue 12 pp 3736—3744

Inhibition of protein kinase D disrupts spindle formation and actin assembly during porcine oocyte maturation

Figure 1. Effects of PKD inhibition on porcine oocyte maturation. (A) The expression of PKD in porcine oocytes. 1, 2, 3 indicates the samples of three replicates. Rabbit monoclonal anti-PKD antibody was adopted. (B) Representative images of cumulus expansion in the control and CID755673-treated groups. Black Crop indicated the status of cumulus expansion in porcine oocytes. Bar = 100 μm (C) Rate of oocytes which extruded the PBI in the control and CID755673 treatment groups, respectively. The rate of PBI extrusion was significantly decreased in 10 μM, 20 μM and 50 μM CID755673-exposed groups. Bar = 30 μm. (D) The proportions of cell cycle distribution were recorded in the control and CID755673 treatment groups. Data were expressed as mean percentage ± s.d. from at least three independent experiments. **, significant difference (P < 0.01), ***, significant difference (P < 0.001).