Research Paper Volume 10, Issue 12 pp 3736—3744

Inhibition of protein kinase D disrupts spindle formation and actin assembly during porcine oocyte maturation

Figure 2. Effects of PKD inhibition on meiotic spindle configurations and chromosome alignment. (A) Representative images of spindle (green) morphologies and chromosome (blue) alignment in the oocytes from control and CID755673 treatment groups. It showed a typical barrel-shaped spindles and well aligned chromosomes in control oocytes; while, unfocused or tripolar poles spindles with agglutinative/scattered chromosomes were successively showed in PKD inhibition groups. Green,α-tubulin; blue, DNA; Bar = 5 μm. (B) The percentage of spindle/chromosome defects were recorded in control and CID755673-treated oocytes. (C) Protein levels of p-MAPK in control and CID755673 treatment oocytes were determined by western blotting. Data are presented as mean ± s.d. from at least three independent experiments. **, significant difference (P < 0.01). ***, significant difference (P < 0.001).