Research Paper Volume 10, Issue 12 pp 3736—3744

Inhibition of protein kinase D disrupts spindle formation and actin assembly during porcine oocyte maturation

Figure 4. PKD1 expression decreases in aged oocytes. (A) Protein levels of PKD1 in control and postovulatory aging porcine oocytes were determined by western blotting. Rabbit polyclonal anti-PKD1 antibody was adopted. (B) Quantitative analysis of PKD1 expression in control and aging groups. Data are presented as mean ± s.d. from at least three independent experiments. **, significant difference (P < 0.01).