Research Paper Volume 10, Issue 12 pp 3774—3793

A CREB1/miR-433 reciprocal feedback loop modulates proliferation and metastasis in colorectal cancer

Figure 3. miR-433 attenuated the invasion and metastasis properties in CRC. (a) Transwell assays showed that restoration or downregulation of miR-433 by transfection of the mimics or inhibitor of miR-433 remarkably rescinded or reinforced the migration and invasion phenotype in LoVo and RKO cells (NC, negative control; mimics, miR-433 mimics; inhibitor NC, negative control for inhibitor; inhibitor, inhibitor of miR-433). (b) Four weeks after intra-splenic injection of LoVo/LV3-miR-433-3p and LoVo/LV3-NC cells, the livers of the two group mice were collected and photographed. (c) Corresponding visible metastatic nodules in the two groups showed that LV3-miR-433-3p conspicuously abolished the metastasis activity of LoVo cells. (d) H&E-stained livers with or without metastases were imaged (met(-), negatively metastatic mouse livers; met(+), positively metastatic mouse livers; T, metastatic tumor; L, liver). *, p<0.05; ***, p<0.001.