Research Paper Volume 10, Issue 12 pp 3794—3805

Sevoflurane impairs learning and memory of the developing brain through post-transcriptional inhibition of CCNA2 via microRNA-19-3p

Figure 2. SEVO impairs neuron cell proliferation in vitro likely through decreasing CCNA2. Identical number of HCV-2 neural cells was exposed to different doses of SEVO for 48 hours and then subjected to analysis. (A-B) Ki-67+ staining on HCN-2 cells, shown by representative images (A), and by quantification (B). (C) The total cell number based on DAPI staining. (D) Western blotting for CDKs and cyclins in 5% SEVO-exposed HCN-2 cells vs gas-exposed HCN-2 cells. (E) Quantification of changes in CCNA2 protein. (F) RT-qPCR for CCNA2 mRNA. *p<0.05. NS: non-significant. N=5. Scale bars are 50µm.