Research Paper Volume 10, Issue 12 pp 3794—3805

Sevoflurane impairs learning and memory of the developing brain through post-transcriptional inhibition of CCNA2 via microRNA-19-3p

Figure 5. Generation of AAVs that express as-miR-19-3p. (A) Schematic showing AAVs carrying as-miR-19-3p under the control of a CMV promoter and AAV carrying null under the control of a CMV promoter as a control. The viral backbone had a GFP reporter, which was co-expressed with the transgene, which allowed the transduced cells to be visualized by green fluorescence. (B) Transduced HCN-2 cell in culture. (C) RT-qPCR for miR-19-3p in AAV-as-miR-19-3p-transduced cells, un-transduced cells (UnT) and null-transduced cells. *p<0.05. NS: non-significant. N=5. Scale bars are 20µm.