Research Paper Volume 10, Issue 12 pp 3806—3820

Circular RNA EIF6 (Hsa_circ_0060060) sponges miR-144-3p to promote the cisplatin-resistance of human thyroid carcinoma cells by autophagy regulation

Figure 7. CircEIF6 knock-down enhanced cisplatin sensitivity of BHT101 cells in vivo. (A and B) Tumor formation curve and tumor weight quantification showed circEIF6 knockdown played a better anti-tumor effect in cisplatin-treated nude mice. *P < 0.05 compared with control and #P < 0.05 compared with NC. (C and D) Increase of miR-144-3p and reduction of TGF-α were detected in circEIF6 knockdown group with cisplatin treatment. *P < 0.05 compared with NC. (E) TGF-α and LC3 II/ LC3 I ratio was lower, while the expression of p62 was much higher in the cisplatin+shCirc-1. *P < 0.05 compared with NC. (F) The cell proliferation marker, Ki67, was detected with Immunohistochemistry, which was significantly fewer with cisplatin treatment and was obviously decreased by circEIF6 knock-down under the cisplatin treatment. *P < 0.05 compared with control and #P < 0.05 compared with NC.