Research Paper Volume 10, Issue 12 pp 3851—3865

Aging-dependent decrease in the numbers of enteric neurons, interstitial cells of Cajal and expression of connexin43 in various regions of gastrointestinal tract

Figure 5. Diminished enteric neurons in the colon of old mice. PGP9.5 recognized perikarya and nerve fibers within MP, and PGP9.5-positive area per field in 20-mo-old mice were significantly declined compared with 2- and 16-mo-old mice (A-B). The reduction in protein expression of PGP9.5 (C) and HuC/D (D) was observed in aging mice consistent with morphological results. Densitometric analysis of protein expressions normalized to α-Tubulin. Statistical analysis was performed using one-way analysis of variance and data were represented as mean ± SD, statistical significance is: (B-D) ## P < 0.01, ### P < 0.001 compared with 2-mo-old group; ** P < 0.01, *** P < 0.001 compared with 16-mo-old group; n=5 mice per group. Abbreviation: Co, colon.