Research Paper Volume 10, Issue 12 pp 3881—3896

Accelerated aging induced by deficiency of Zmpste24 protects old mice to develop bleomycin-induced pulmonary fibrosis

Figure 7. Dysregulated miRNA expression and gene targets of bleomycin-treated lungs of old Zmpste24 deficient mice (n=3) compared to old WT (n=3) analyzed by quantitative RT-PCR. (A) Pie graph showing significantly different mRNA (up= 627, down= 538) and miRNAs (up= 22, down= 20) in lungs of Zmpste24 deficient mice compared with their WT counterparts after bleomycin damage. (B) Expression levels of selected miRNAs. Gene expression of (C) miR29 targets, (D) miR27a targets, (E) mir145a targets. White bars represent mean expression in WT lungs, and black bars represent mean expression in Zmpste24 deficient mice ± S.E.M. *p < 0.05 and ** p< 0.01.