Research Paper Volume 10, Issue 12 pp 3897—3909

Melatonin prevents deterioration in quality by preserving epigenetic modifications of porcine oocytes after prolonged culture

Figure 1. Melatonin enhances the blastocyst formation rate of prolonged-culture oocytes. Porcine oocytes matured in vitro were continuously cultured in medium supplemented with or without melatonin (10−3 or 10−5 M) for 24 h. Oocytes were pathogenetically activated and then cultured for 7 days to examine the blastocyst formation rate. Views of blastocysts (A) and blastocyst formation rate (B) of fresh, prolonged-culture, and prolonged-culture + Mel oocytes. The data are presented as the mean ± SEM of at least three independent experiments. Bar=200 μm; PC, prolonged-culture; Mel, melatonin; *P<0.05, **P<0.01.